Method of enzymatically synthesizing 3′-phosphoadenosine-5′-phosphosulfate

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United States of America Patent

PATENT NO 9193958
APP PUB NO 20140206042A1
SERIAL NO

14199337

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Abstract

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The invention provides a method for producing 3′-phosphoadenosine 5′-phosphosulfate (PAPS), the method including subjecting ATP to sulfation and phosphorylation by use of adenosine 5′-triphosphate sulfurylase (ATPS) and adenosine 5′-phosphosulfate kinase (APSK), wherein an adenosine 5′-triphosphate (ATP) supply/regeneration system including adenosine 5′-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5′-diphosphate kinase (PNDK), and polyphosphate:AMP phosphotransferase (PAP), or an adenosine 5′-triphosphate (ATP) supply/regeneration system including adenosine 5′-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5′-diphosphate kinase (PNDK), and adenylate kinase (ADK) is employed instead of ATP.

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Patent Owner(s)

Patent OwnerAddress
YAMASA CORPORATIONCHOSHI-SHI CHIBA 288-0056

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Ishige, Kazuya Choshi, JP 17 9
Noguchi, Toshitada Choshi, JP 19 19

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