, methods for producing and purifying Sm-14 and the use thereof as a vaccine and diagnostic medium
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United States of America Patent
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Nov 24, 2015
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Sep 12, 2013
app pub date -
Sep 13, 2011
filing date -
Sep 17, 2010
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Abstract
The production of recombinant proteins using a synthetic gene for increased expression of the protein in Pichia pastoris is disclosed. More specifically, disclosed is a process for producing a recombinant Sm14 protein of Schistosoma mansoni, by providing a synthetic gene for increased production of Sm14 protein, producing the gene and genetically manipulating the Pichia pastoris strain with the gene in order to produce a vaccine effectively. Improved methods are also provided for producing and purifying this protein from P. pastoris cells, which can be scaled for industrial production.
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