Hybridoma cell line ST03, monoclonal antibody against aflatoxin biosynthetic precursor sterigmatocystin and use thereof

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United States of America Patent

PATENT NO 9176136
APP PUB NO 20150276734A1
SERIAL NO

14666869

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A hybridoma cell line ST03 having China Center for Type Culture Collection (CCTCC) accession number C2013187, a monoclonal antibody against aflatoxin biosynthetic precursor ST produced by the hybridoma cell line ST03, and the use of the monoclonal antibody. The hybridoma cell line ST03 can be used for preparing a high-titer monoclonal antibody against aflatoxin biosynthetic precursor ST, and the titer of mouse ascites antibody against aflatoxin biosynthetic precursor ST determined by enzyme linked immunosorbent assay (ELISA) can reach 6.4×105. The monoclonal antibody against aflatoxin biosynthetic precursor ST has high sensitivity, has 50% inhibiting concentration IC50 to aflatoxin biosynthetic precursor ST of 0.36 ng/mL, has no cross reaction with all of aflatoxin B1, aflatoxin B2, aflatoxin G1, and aflatoxin G2, and can be used for the content determination of aflatoxin biosynthetic precursor ST.

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Patent Owner(s)

Patent OwnerAddress
OILCROPS RESEARCH INSTITUTE OF CHINESE ACADEMY OF AGRICULTURE SCIENCESWUCHANG WUHAN HUBEI 430062

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Ding, Xiaoxia Hubei, CN 13 6
Li, Min Hubei, CN 751 7676
Li, Peiwu Hubei, CN 32 10
Zhang, Qi Hubei, CN 768 6016
Zhang, Zhaowei Hubei, CN 13 3

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