Process for culturing adherent cells
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United States of America Patent
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Sep 29, 2015
Grant Date -
Jun 23, 2011
app pub date -
Dec 22, 2010
filing date -
Dec 23, 2009
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Abstract
The subject of the present invention is a process for producing adherent cells comprising:
- a. introducing a suspension of adherent cells into a culture vessel containing microcarriers in a culture medium;b. amplifying the cells by performing a plurality of cell passages in the same culture vessel wherein each cell passage subsequent to the first is carried out: i) by using all or part of the cells produced during the previous cell passage after having subjected the cells to enzyme treatment to detach the cells from the microcarriers, andii) by introducing culture medium and an increasing amount of microcarriers into the culture medium; and c. harvesting the cells produced during the last cell passage, optionally after having subjected the cells to enzyme treatment to detach cells from microcarriers.
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