Method of fluorescence imaging
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United States of America Patent
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Sep 15, 2015
Grant Date -
Nov 4, 2010
app pub date -
May 30, 2008
filing date -
Jun 1, 2007
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Abstract
Method of fluorescence imaging including: illuminating a sample to excite its fluorescence and acquiring an image thereof; based on fluorescence spectral and spatial information from the sample's fluorescence image, segmenting the image into regions of similar spectral properties; for each image segment, exciting the fluorescence of the corresponding sample region, and detecting the corresponding fluorescence; based on modelling, determining expected fluorescence parameters from the fluorescence signals detected for each region; scanning the sample and determining final fluorescence parameters based on said expected fluorescence parameters.
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