Step-up method for COLD-PCR enrichment
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United States of America Patent
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Sep 15, 2015
Grant Date -
Nov 21, 2013
app pub date -
Mar 15, 2013
filing date -
May 16, 2012
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Abstract
Methods of using polymerase chain reactions to enrich a target sequence in a sample containing reference sequences and target sequences having high homology and amplifiable by the same primer pair are provided herein. In particular the methods provide a robust means to improve the fold enrichment of the target sequence and minimize reaction-to-reaction, well-to-well and run-to-run variations in the enrichment methods.
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