High-speed maturation method for an oligonucleotide library for the purpose of preparing a protein library
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United States of America Patent
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Sep 15, 2015
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Jun 6, 2013
app pub date -
Jun 23, 2011
filing date -
Jun 23, 2010
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Abstract
The present invention provides a method of producing a maturated oligonucleotide library, including a step of obtaining a terminal-modified product of a maturation target oligonucleotide library, including adding a tag sequence to the 5′ terminus of the maturation target oligonucleotide library and an arrest sequence, which stalls translation elongation on a ribosome, to the 3′ terminus of the maturation target oligonucleotide library, a step of transcribing the terminal-modified sequence product to give a transcript, and a step of in vitro translation for translating the transcript in vitro, wherein the maturation target oligonucleotide library is a random oligonucleotide library.
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