Method of removing nucleic acid contamination in reverse transcription and amplification reactions
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United States of America Patent
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Sep 15, 2015
Grant Date -
Apr 3, 2014
app pub date -
Sep 25, 2013
filing date -
Jul 21, 2009
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Abstract
The invention provides methods of removing nucleic acid contamination from reverse transcription reactions and hot-start PCR, wherein said hot-start PCR is a barrier hot-start PCR set up and/or involves a hot-start DNA polymerase, which methods comprise use of a DNase that is substantially irreversibly inactivated by heating at a temperature of about 50° C. for 5 minutes, and that is substantially specific for double stranded DNA. The invention further provides a DNase that is substantially irreversibly inactivated by heating at a temperature of about 50° C. for 5 minutes, and that is substantially specific for double stranded DNA, nucleic acids encoding said DNase and kits or compositions comprising said DNase or said nucleic acid.
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