Methods and compositions for discrimination between cytosine and modifications thereof and for methylome analysis
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United States of America Patent
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Sep 1, 2015
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app pub date -
Mar 14, 2013
filing date -
Mar 15, 2012
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Abstract
Compositions and methods are provided for discrimination between cytosine and modifications thereof using cytidine deaminases and/or oxygenases. Variants of wild type cytidine deaminases are described which show reduced bias with respect to adjacent nucleotides upstream of the cytosine. The methods provide a rapid and convenient use of enzymes to obtain methylomes.
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