Method of determining the abundance of a target nucleotide sequence of a gene of interest
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United States of America Patent
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Sep 1, 2015
Grant Date -
Jun 6, 2013
app pub date -
Oct 31, 2012
filing date -
Oct 31, 2011
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Abstract
The disclosure relates to a method of measuring gene abundance, including obtaining at least two types of amplification product, each of which contains a single additional base sequence and corresponds to each of at least two genes, by amplifying, in one reaction solution, nucleic acids encoding the at least two genes, whose abundances in nucleic acids contained in a subject sample may be different, using a first primer set, which includes at least two types of first primer, each of which is capable of introducing the single additional base sequence to a resulting amplification product and corresponds to each of the at least two genes, and a second primer for amplifying a nucleic acid containing the single additional base sequence; and determining the abundances of the at least two genes based on detected signals corresponding to the abundances of the at least two types of amplification product.
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