Method for production of lactoferrin
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United States of America Patent
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Aug 25, 2015
Grant Date -
Dec 8, 2011
app pub date -
Jan 28, 2010
filing date -
Jan 28, 2009
priority date (Note) -
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status (Latency Note)
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Abstract
The invention concerns a method for production of lactoferrin comprising at least the steps of: a) disposing of raw material that have not been treated at a temperature greater than 500C, b) submitting this raw material to a treatment in order to obtain a solution of Lactenin (LN) or Milk Basic Protein (MBP), c) submitting this LN or MBP solution to a step of purification on a cation exchange resin equilibrated with an acetate buffer at a pH between 4 and 9 and eluted with different buffer solutions containing different solute concentrations, d) and collecting a fraction containing Lactoferrin having more than 95% of purity, having no polymers and substantially free of LPS, endotoxins and angiogenin. It also concerns the Lactoferrin obtained having more than 95% of purity, substantially free of LPS, endotoxins and angiogenin with an iron saturation level comprised between 9% to 15%.
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International Classification(s)
- A61K:PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOS....
- A01N:PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLA....
- A23L:FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT....
- C12N:MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF
- A23J:PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PR....
- C07K:PEPTIDES
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