Method for quantifying modified peptides
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United States of America Patent
Stats
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Aug 18, 2015
Grant Date -
Mar 22, 2012
app pub date -
Apr 16, 2010
filing date -
Apr 17, 2009
priority date (Note) -
In Force
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Abstract
The present invention provides a method for quantifying modified peptides in a sample, the method comprising: (a) obtaining peptides from the sample; (b) adding reference modified peptides to the peptides obtained in step (a) to produce a mixture of peptides and reference modified peptides; (c) carrying out mass spectrometry (MS) on said mixture of peptides and reference modified peptides to obtain data relating to the peptides in the sample; and (d) comparing the data relating to the peptides in the sample with data in a database of modified peptides using a computer program; wherein the database of modified peptides is compiled by a method comprising: (i) obtaining peptides from a sample; (ii) enriching modified peptides from the peptides obtained in step (i); (iii) carrying out liquid chromatography-tandem mass spectrometry (LC-MS/MS) on the enriched modified peptides obtained in step (ii); (iv) comparing the modified peptides detected in step (iii) to a known reference database in order to identify the modified peptides; and (v) compiling data relating to the modified peptides identified in step (iv) into a database.

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Patent Owner(s)
Patent Owner | Address | |
---|---|---|
HVIVO SERVICES LIMITED | QUEEN MARY BIOENTERPRISES INNOVATION CENTRE 42 NEW ROAD LONDON E1 2AX |
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Inventor(s)
Inventor Name | Address | # of filed Patents | Total Citations |
---|---|---|---|
Rodriguez, Cutillas Pedro | London, GB | 4 | 1 |
Vanhaesebroeck, Bart | London, GB | 9 | 127 |
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