Methods for compact aggregation of dermal cells
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United States of America Patent
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Aug 18, 2015
Grant Date -
Dec 2, 2010
app pub date -
Feb 28, 2007
filing date -
Feb 28, 2006
priority date (Note) -
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Abstract
The invention provides for a method for aggregating dermal papilla cells or dermal sheath cells or a combination thereof, the method comprising: growing dermal papilla cells or dermal sheath cells or a combination thereof in suspension culture; and contacting the culture with an effective amount of an enzyme, wherein a substrate of the enzyme is an extracellular matrix molecule in the suspension culture, so as to aggregate dermal papilla cells or dermal sheath cells. The culture may be a hanging drop culture and the enzyme may be a hyaluronidase.
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