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United States of America Patent
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Aug 11, 2015
Grant Date -
Jul 1, 2010
app pub date -
Oct 2, 2008
filing date -
May 23, 2002
priority date (Note) -
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Abstract
Genetically modified strains of C. tropicalis, which will not revert to wild-type activity at the POX 4 and/or POX 5 locus, are disclosed. The strains are β-oxidation blocked and have been transformed through homologous recombination with a construct which deletes a portion of the POX 4 and/or POX 5 gene. The modified strains may be used to increase yields of dicarboxylic acids produced in host cells of the strains. Methods for blocking the β-oxidation pathway in a C. tropicalis host cell are also provided.
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