Process for production of protein
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United States of America Patent
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Aug 11, 2015
Grant Date -
Aug 2, 2012
app pub date -
Dec 15, 2011
filing date -
Dec 15, 2010
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Abstract
The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; and a method for producing the protein of interest comprising suspension-culturing a suspension mammalian cell which produces the protein of interest; and an a suspension mammalian cell which expresses the protein of interest.
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