Sequence of porcine ROSA26 locus and methods of using the same
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United States of America Patent
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Jul 28, 2015
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Nov 6, 2014
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May 4, 2013
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May 4, 2012
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Abstract
The present invention provides a 5 kb sequence of porcine ROSA26 locus, which can be used for site-specific integration of an exogenous gene into a pig genome. The present invention also provides a gene targeting vector with a 3′ and 5′ arm of the porcine ROSA26 sequence, wherein a target gene can be inserted between the 3′ and 5′ arm sequence. The gene targeting vector can be used to generate transgenic pigs with stable and ubiquitous expression of the target gene.
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