Process for preparation of insulin compounds
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United States of America Patent
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Jul 28, 2015
Grant Date -
Jun 30, 2011
app pub date -
Sep 19, 2008
filing date -
Aug 7, 2008
priority date (Note) -
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Abstract
The present invention relates to the preparation of insulin compounds including their analogs or derivatives thereof from their corresponding precursor forms by a one step enzymatic reaction involving the combinatorial and concurrent use of optimal quantities of trypsin and carboxypeptidase B that work synergistically directing the reaction in a controlled manner to avoid production of random undesired byproducts. Particularly, the enzymatic conversion reactions of the instant invention offer advantages of reduction in the number operational steps, higher yield and purity of the desired end products.
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