Compositions and methods using split polymerases
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United States of America Patent
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Jul 21, 2015
Grant Date -
Sep 18, 2008
app pub date -
Dec 31, 2007
filing date -
Dec 29, 2006
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Abstract
The invention relates to compositions and methods utilizing split polymerase enzymes composed of at least two discrete polypeptides that stably associate to form a single polymerase. The invention further relates to nucleic acid constructs for expressing the split polymerases of the invention, and methods for using the split polymerases of the invention. The enzymes of the invention are useful in many applications calling for the detectable labeling of nucleic acids and are particularly useful in quantitative PCR (QPCR) and DNA sequencing applications.
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