Culturing human embryonic stem cells with a noggin to generate cells lacking Pax-6 expression
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United States of America Patent
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Jul 14, 2015
Grant Date -
Aug 6, 2009
app pub date -
Jan 30, 2009
filing date -
Jun 20, 2000
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Abstract
The present invention provides a preparation of undifferentiated embryonic stem (ES) cells sustainable for a prolonged period in an undifferentiated state which will undergo stem cell renewal or somatic differentiation. Preferably the cells are capable of somatic differentiation in vitro and are inclined to differentiate away from an extraembryonic lineage. The present invention also provides method of culturing embryonic stem (ES) cells to improve stem cell maintenance and persistence in culture. The method also provides a culture of ES cells prepared by the method as well as differentiated cells derived from the embryonic cells resulting from directed differentiation procedures provided by the present invention.
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