Method of regenerating elastic fiber with the use of dance or factor enhancing the expression thereof
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United States of America Patent
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Jul 7, 2015
Grant Date -
Jan 8, 2009
app pub date -
Jan 23, 2006
filing date -
Feb 7, 2005
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Abstract
The present invention provides a technique for conveniently and efficiently regenerating elastic fiber retaining a normal structure, and a technique enabling preparation of an artificial tissue comprising elastic fiber (for example, artificial skin, artificial blood vessels) that can be transplanted to humans. Specifically, the invention provides a method of producing elastic fiber, comprising culturing (for example, culturing in serum-free medium) cells having the capability of regenerating elastic fiber in the presence of DANCE and/or fibulin-4; artificial elastic fiber comprising DANCE and/or fibulin-4; an elastic fiber regenerating agent; a serum-free medium comprising DANCE and/or fibulin-4; and cells having the capability of regenerating elastic fiber, transfected with at least one of a DANCE expression vector, a fibulin-4 expression vector and a DANCE inducing factor expression vector, and the like.
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