Method for enhancing recombinant protein production by cell-free protein expression system
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United States of America Patent
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May 26, 2015
Grant Date -
Oct 27, 2011
app pub date -
Apr 14, 2011
filing date -
Apr 14, 2010
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Abstract
The present invention provides a method for monitoring of profile changes of components in a dynamic system such as a cell-free in vitro protein synthesis system by using liquid chromatography (LC) combined with mass spectrometry (MS). In an additional aspect, this invention provides a method for enhancing the yield and/or reproducibility in a cell-free protein synthesis system by modulating the level and/or activity of a protein component that has regulatory effects on the system.
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