Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains
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United States of America Patent
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Apr 7, 2015
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Mar 26, 2014
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Dec 12, 2012
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Abstract
The invention provides for engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors with additional functional domains. Also provided are methods of directing CRISPR complex formation in prokaryotic and eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity.
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