Gene expression technique
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United States of America Patent
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Mar 3, 2015
Grant Date -
Sep 13, 2012
app pub date -
May 17, 2012
filing date -
Dec 23, 2003
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Abstract
The present invention provides a method for producing a desired protein (such as a desired heterologous protein) comprising:
- (a) providing a host cell comprising a first recombinant gene encoding a protein comprising the sequence of a first chaperone protein, a second recombinant gene encoding a protein comprising the sequence of a second chaperone protein and a third gene, such as a third recombinant gene, encoding a desired protein (such as a desired heterologous protein), wherein the first and second chaperones are different; and(b) culturing the host cell in a culture medium to obtain expression of the first, second and third genes.
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