Production cell line
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United States of America Patent
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Feb 24, 2015
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app pub date -
Jul 4, 2011
filing date -
Jul 5, 2010
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Abstract
The invention refers to a method of producing a recombinant polypeptide of interest (POI) in a cell culture, comprising genetically engineering a eukaryotic cell line—to specifically cause prolongation of the G2+M cell cycle phase in a pre-culture phase, and—to produce the POI in a producing phase following the pre-culture phase, a high producer cell line and cell culture as well as a method of increasing the yield of a recombinant POI production in a cell culture.
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