Methods of cell culture for adoptive cell therapy

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United States of America Patent

PATENT NO 8956860
SERIAL NO

13475700

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Abstract

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An improved method of culturing cells for cell therapy applications that includes growing desired cells in the presence of antigen-presenting cells and/or feeder cells and with medium volume to surface area ratio of up to 1 ml/cm2 if the growth surface is not comprised of gas permeable material and up to 2 ml/cm2 if the growth surface is comprised of gas permeable material. The desired cells are at a surface density of less than 0.5×106 cells/cm2 at the onset of a production cycle, and the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25×105 cells/cm2.

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Patent Owner(s)

Patent OwnerAddress
WILSON WOLF MANUFACTURING33 5TH AVENUE NORTHWEST SUITE 700 NEW BRIGHTON MN 55112

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Leen, Ann M Bellaire, US 14 738
Rooney, Cliona M Bellaire, US 46 967
Vera, Juan F Bellaire, US 16 741
Wilson, John R New Brighton, US 170 9289

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