Construction of Highly efficient cellulase compositions for enzymatic hydrolysis of cellulose

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United States of America Patent

PATENT NO 8916363
SERIAL NO

12908454

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Abstract

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This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, β-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.

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Patent Owner(s)

Patent OwnerAddress
DANISCO US INC925 PAGE MILL ROAD PALO ALTO CA 94304

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Antonov, Alexey I Moscow, RU 3 74
Baez, Marco A Jupiter, US 5 81
Burlingame, Richard P Jupiter, US 19 312
Emalfarb, Mark A Jupiter, US 11 284
Gusakov, Alexander V Moscow, RU 3 74
Okunev, Oleg N Moscow Region, RU 3 74
Salanovich, Tatyana N Moscow, RU 3 74
Sinitsyn, Arkady P Moscow, RU 3 74
Ustinov, Boris B Tula, RU 3 74

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