Kit and method for determining whether or not unmethylated cytosine conversion treatment is properly carried out and method for analyzing methylated DNA using the same
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United States of America Patent
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Dec 9, 2014
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Mar 16, 2009
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Mar 31, 2008
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Abstract
Success or failure of unmethylated cytosine conversion treatment is determined by using a first primer set comprising plural primers that hybridize with a nucleic acid comprising a nucleotide sequence not containing cytosine in the nucleotide sequence of biological DNA that is subject to an unmethylated cytosine conversion treatment and a second primer set comprising plural primers that hybridize with a nucleic acid comprising a nucleotide sequence in which cytosine in a nucleotide sequence containing cytosine and not containing a CpG site is converted into a base other than cytosine, in the nucleotide sequence of biological DNA.
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