Gel for isoelectric focusing
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United States of America Patent
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Oct 14, 2014
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app pub date -
Feb 10, 2012
filing date -
Dec 22, 2005
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Abstract
The present invention relates to the field of electrophoresis and more specifically to a gel or strip for separating peptide components by isoelectric focusing by producing IPG (immobilized pH gradient) gels or strips in a novel way before focusing. More closely, the peptides are focused in a novel IPG gel including an uneven or non-linear pH gradient having at least three separate stepwise arranged pH-intervals. After focusing the peptide resolution is high and the peptides are evenly distributed along the gel.
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