Mycobacteria culture medium and method including mycobacteria of mycobacterium tuberculosis complex
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United States of America Patent
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Sep 23, 2014
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Oct 20, 2009
filing date -
Dec 5, 2008
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Abstract
The present invention relates to a novel mycobacteria culture medium, particularly for mycobacteria of the Mycobacterium tuberculosis complex, that significantly reduces the culture isolation time and thus the time for diagnosing the mycobacteria, particularly those for tuberculosis. A culture medium according to the invention contains defibrinated blood, lecithin, and decomplemented fetal calf serum. The present invention also relates to a culture method and to a method for identifying mycobacteria, particularly bacteria of the Mycobacterium tuberculosis complex. The present invention also relates to a novel decontamination method through chlorhexidine treatment of biological samples in an isolation and mycobacteria culture medium, and to a method for determining by phenotype the sensitivity of mycobacteria to antibiotics by means of a solid culture medium according to the invention. Finally, the present invention also relates to a method of identification in liquid phase by mass spectrometry which reduces time for diagnosing mycobacteriosis, particularly for tuberculosis.
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