Method for detection and/or analysis of yeast and mold in filterable liquids
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United States of America Patent
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Sep 16, 2014
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app pub date -
Nov 12, 2008
filing date -
Nov 16, 2007
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Abstract
This invention is a process for preparing a food or beverage sample containing yeast or mold cells for analytical testing. The food sample is prepared into the form of a filterable liquid, and then filtered using a glass microfiber filter. The filter containing the fungal cell retentate is then placed into a disruption vessel and bead beaten until the glass microfiber filter is completely disrupted into glass fibers in suspension. An aliquot can then be tested directly using melting curve analysis of PCR amplification product derived from the nucleic acids of the sample to detect the presence of the fungal cells from the sample.
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