Method for protein isolation in anoxic conditions

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United States of America Patent

PATENT NO 8802395
APP PUB NO 20130150553A1
SERIAL NO

13467305

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Abstract

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The present invention relates to a method for the isolation of proteins that comprise disulfide bonds in their native conformation. Essentially, a method of the present invention makes use of reducing agents such as β-mercaptoethanol or dithiothreitol in protein isolation methods obsolete. A method of the present invention is particularly suitable for the isolation of precursor proteins such as proinsulin from recombinant cells.

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Patent Owner(s)

Patent OwnerAddress
MERCK SHARP & DOHME B V2031 BN HAARLEM

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Eppink, Michel Hendrikus Maria Oss, NL 4 4
Van, Dedem Gijsbert Willem Karel Oss, NL 3 1
Van, Der Meijden Peter Oss, NL 9 8
Wassenaar, Roeland Wilhelmus Oss, NL 3 1

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