Method for protein isolation in anoxic conditions
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United States of America Patent
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Aug 12, 2014
Grant Date -
Jun 13, 2013
app pub date -
May 9, 2012
filing date -
Jan 31, 2003
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Abstract
The present invention relates to a method for the isolation of proteins that comprise disulfide bonds in their native conformation. Essentially, a method of the present invention makes use of reducing agents such as β-mercaptoethanol or dithiothreitol in protein isolation methods obsolete. A method of the present invention is particularly suitable for the isolation of precursor proteins such as proinsulin from recombinant cells.
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