Culture medium and method for inducing differentiation into adipocytes
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United States of America Patent
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Jul 22, 2014
Grant Date -
Dec 15, 2011
app pub date -
Feb 18, 2010
filing date -
Feb 23, 2009
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Abstract
Disclosed are a culture medium, an additive, and a method for efficiently inducing the differentiation of mammalian somatic stem cells into cells having the characteristics of adipocytes under conditions of serum-free or low-serum culture medium. The culture medium for inducing the differentiation of mammalian somatic stem cells into adipocytes comprises a basal medium for culturing mammalian cells, an agent for inducing the differentiation of mammalian somatic stem cells into adipocytes, biotin, a ligand for endothelial cell differentiation gene (Edg) family receptors, vitamin C, and HEPES, which culture medium is serum-free or contains a low concentration of serum.
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