Method of elongating DNA through immobilizing primer DNA chains on a substrate, a method of amplifying a DNA chain
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United States of America Patent
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Jul 1, 2014
Grant Date -
Feb 7, 2008
app pub date -
Nov 29, 2005
filing date -
Dec 9, 2004
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Abstract
Primers for DNA elongation are immobilized onto a substrate having on the surface thereof a polymer substance which contains a first unit having a group derived from a phosphoester composing the hydrophilic section of a phospholipid, and a second unit having an activated ester group, and DNA elongation reaction is allowed to proceed in a reaction system having introduced therein a sample which contains DNA fragments having desired sequences and nucleotide monomers, by heating the reaction system up to a temperature causing thermal unfolding of the DNA chains, and then by cooling the reaction system down to a temperature for annealing.
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