Inhibition-based high-throughput screen strategy for cell clones
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United States of America Patent
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Jul 1, 2014
Grant Date -
Dec 16, 2010
app pub date -
Jun 3, 2010
filing date -
Jun 11, 2009
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Abstract
A method for screening cells with high level expression of a target protein is disclosed. The method includes introducing into a plurality of host cells a DNA construct that encodes both a target protein and an inhibitor to an endogenous selectable marker in the host cells, screening host cells harboring the DNA construct for the expression of the endogenous selectable marker, and isolating cells with reduced expression of the selectable marker. Also disclosed is a DNA construct configured to express both the target protein and the inhibitor inside the host cell.
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