Quantitative nuclease protection assay (QNPA) and sequencing (QNPS) improvements
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United States of America Patent
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Jun 3, 2014
Grant Date -
Mar 27, 2014
app pub date -
Apr 26, 2012
filing date -
May 4, 2011
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Abstract
The present disclosure provides an improvement to quantitative Nuclease Protection Assay (qNPA) and quantitative Nuclease Protection Sequencing (qNPS) methods. The disclosed methods use nuclease protection probes (NPPs) that include 5′-end and/or 3-end flanking sequences, which provide a universal hybridization and/or amplification sequence. The disclosed methods can be used to sequence or detect target nucleic acid molecules, such as those present in fixed or insoluble samples.
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