Transformed strains originated from multidrug efflux protein defective strains and a method for microbial conversion using them
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United States of America Patent
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May 27, 2014
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Feb 16, 2012
app pub date -
Feb 28, 2008
filing date -
Mar 1, 2007
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Abstract
Disclosed is a means for improving the poor conversion efficiency in a conventional bioconversion system using a transformant which is given by introducing a gene originated from xerogenic organisms. A transformant is prepared by using a host which is defective in a gene encoding a multidrug efflux protein and introducing a gene originated from xerogenic organisms. Use of the transformant results in much effective microbial conversion of a hydrophobic or amphipathic substrate compound into a desired compound. In case, an Escherichia coli is used as the host, the gene encoding a multidrug efflux protein to be defective may be tolC, acrA, acrB and the like.
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