Generation of random double-strand breaks in DNA using enzymes
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United States of America Patent
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Apr 22, 2014
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Oct 20, 2011
app pub date -
Feb 3, 2010
filing date -
Feb 3, 2009
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Abstract
An enzyme preparation is described that includes a non-specific nuclease and a T7 Endo I mutant in a unit ratio of less than 1:200. This enzyme preparation may be used to generate double-stranded DNA fragments of a size suitable for DNA sequencing. The ends of the fragments can be readily modified as necessary to ligate adaptors or individual nucleotides to one strand of the double-stranded DNA fragments.
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