Methods, plasmid vectors and primers for assessing HIV viral fitness
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United States of America Patent
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Mar 18, 2014
Grant Date -
Feb 11, 2010
app pub date -
Dec 7, 2006
filing date -
Dec 7, 2005
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Abstract
The present invention relates to methods and means for the evaluation of HIV replicative capacity in a given environment. In particular, the invention provides a growth competition assay that can determine relative viral fitness using a recombinant tagged HIV-1 virus system. The methods rely on plasmid vectors, amplicons, primers and probes, and the generation of replication-competent viruses therefrom. Said methods and materials may find use in multiple fields including diagnostics, drug screening, pharmacogenetics and drug development.
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