strain, mutagenesis and screening methods thereof, and methods of fermenting to produce fumaric acid

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United States of America Patent

PATENT NO 8663972
APP PUB NO 20140004560A1
SERIAL NO

13992233

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A rhizopus oryzae strain, mutagenesis and screening methods thereof, and methods of producing fumaric acid by fermentation. The strain is named as Rhizopus oryzae ME-F13, and deposited in China Center for Type Culture Collection with depository number CCTCC M 2010351. The strain is obtained by physically mutagenizing the original strain ME-F12 through ion injection, culturing the processed bacteria on the solid selective plate containing 2-D-deoxylucose (2-DG) and picking up 2-DG-resistant single colony. The strain is capable of simultaneously saccharifying starchy material and fermenting it to produce fumaric acid. With an improved enzymatic activity, the strain can be directly used to ferment raw starchy materials without needing pre-saccharifying.

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Patent Owner(s)

Patent OwnerAddress
ANHUI SEALONG BIOTECHNOLOGY CO LTD233000 NO 6 JINYI ROAD MOHEKOU INDUSTRIAL PARK HUAISHANG DISTRICT BENGBU CITY ANHUI PROVINCE BENGBU CITY ANHUI PROVINCE 233000

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Gao, Zhen Jiangsu, CN 17 48
Huang, He Jiangsu, CN 482 2786
Li, Shuang Jiangsu, CN 159 633
Xu, Qing Jiangsu, CN 228 2805

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