Cascade enzyme-linked immunosorbent assay
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United States of America Patent
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Feb 4, 2014
Grant Date -
Aug 5, 2010
app pub date -
May 30, 2008
filing date -
Oct 1, 2007
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Abstract
The present invention relates to a cascade enzyme-linked immunosorbent assay, more precisely a cascade enzyme-linked immunosorbent assay using magnetic microparticles (MMPs) immobilized with the target antigen specific primary antibody and silica nanoparticles (SPs) immobilized with a cascade reaction initiator and the antigen-specific secondary antibody. When the method of the present invention is applied in the detection of an antigen in biosamples, the detection sensitivity can be significantly increased.
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