Solubilization and purification of a target protein fused to a mutant maltose-binding protein
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United States of America Patent
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Jan 7, 2014
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Feb 2, 2012
app pub date -
Apr 1, 2009
filing date -
Apr 1, 2009
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Abstract
Methods and compositions are provided that relate to a composition that includes a modified maltose-binding protein (MBP) which when fused to a protein results in an increase in binding affinity for maltodextrin compared with the wild type MBP fused to the protein, the modified MBP maintaining enhanced solubility. The modification includes a mutation selected from the group consisting of: F68L, I318V, Q326R, V344M, and T372TTTITITTTLGIEGR387 or consists of two or more mutations selected from the group consisting of: F68L, S146T, A313V, I318V, I318A, Q326R, V344M and T372TTTITITTTLGIEGR387 mutants.
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