Method for preparing nucleotides and related analogues by synthesis on soluble substrate, and biological tools thus prepared
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United States of America Patent
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Dec 24, 2013
Grant Date -
May 19, 2011
app pub date -
Feb 23, 2009
filing date -
Feb 21, 2008
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Abstract
The invention concerns a method for preparing monomer nucleotides or nucleotide analogues comprising the steps of: (1) coupling a soluble polyethylene glycol support provided with at least one diacid or ether-acid linker and a monomer nucleoside or nucleoside analogue to an amine group or hydroxyl group of the nucleoside with the aid of a coupling agent; (2) at least one step for phosphorylation of said nucleoside or nucleoside analogue coupled to said support with a phosphorylation agent; (3) cleavage of said support and recovery of at least one monomer nucleotide or nucleotide analogue. The nucleotides prepared are biological tools.
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