Bioassay method for yokukansan with serotonin receptor
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United States of America Patent
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Dec 17, 2013
Grant Date -
Dec 16, 2010
app pub date -
Feb 15, 2008
filing date -
Feb 15, 2008
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Abstract
The invention intends to find out an in-vitro bioassay system capable of ensuring qualities of yokukansan to a higher degree, and provides a bioassay method for yokukansan, comprising competitively reacting labeled ligand and a test sample containing yokukansan with cells or cell membranes expressing serotonin 1A receptors, and measuring binding activity of yokukansan from the amount of the labeled ligand bound, and a bioassay method for yokukansan, comprising reacting labeled GTP and a test sample containing yokukansan with cells or cell membranes expressing serotonin 1A receptors, and measuring receptor-agonist activity of yokukansan from the amount of the labeled GTP bound.
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