Methods and systems for image processing of microfluidic devices
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United States of America Patent
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Dec 3, 2013
Grant Date -
Sep 27, 2012
app pub date -
Oct 5, 2011
filing date -
Sep 13, 2006
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Abstract
A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.
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