Methods for simplifying microbial nucleic acids by chemical modification of cytosines
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United States of America Patent
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Dec 3, 2013
Grant Date -
Jun 9, 2011
app pub date -
Sep 28, 2010
filing date -
Dec 3, 2004
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Abstract
A method for detecting a microorganism comprising reducing the complexity of a microbial genome or microbial nucleic acid by generating a simplified form of the microbial genome or microbial nucleic acid in which substantially all of the positions naturally occupied by cytosines are occupied by uracil or thymine; and assaying for a microbial specific nucleic acid in the simplified form of the microbial genome or microbial nucleic acid, wherein presence of the microbial specific nucleic acid is indicative of the microorganism.
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