by targeting cytolethal distending toxin

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United States of America Patent

PATENT NO 8586327
APP PUB NO 20100273161A1
SERIAL NO

12675726

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Abstract

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An objective of the present invention is to provide the cytolethal distending toxin (CDT) of C. hyointestinalis and polynucleotides encoding it, and novel methods for detection of C. hyointestinalis using the cdt genes. The present inventors focused on the cytolethal distending toxin (CDT) of Campylobacter bacteria, and detected the cdt genes of a Campylobacter-like bacterium isolated from an enteritis patient in Thailand. The present inventors discovered a bacterial strain whose cdtB gene was amplified by common primers in C. jejuni, C. coli, and C. fetus, but not by multiplex PCR that can specifically detect the cdtA, cdtB, and cdtC genes of the three bacterial species. The bacterial strain was identified as C. hyointestinalis by 16S rRNA gene analysis. Furthermore, the entire nucleotide sequence of the cdt genes was determined by genome walking upstream and downstream of the cdtB gene.

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Patent Owner(s)

Patent OwnerAddress
OSAKA PREFECTURE UNIVERSITYSAKAI-SHI OSAKA 5998531

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Asakura, Masahiro Osaka, JP 17 68
Samosorunsuk, Worada Sakai, JP 2 2
Yamasaki, Shinji Sakai, JP 41 299

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