Method for detecting and quantifying poly(A) RNA and mRNA
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United States of America Patent
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Nov 19, 2013
Grant Date -
Aug 30, 2012
app pub date -
Dec 1, 2010
filing date -
Dec 4, 2009
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Abstract
The present invention relates to a method for detecting and/or for quantifying poly(A) RNA and/or mRNA, wherein a poly(dT) oligonucleotide which features a fluorescent dye and also a quencher is hybridized to the nucleic acid to be detected. Non-hybridized poly(dT) oligonucleotide is degraded by an added nuclease, and as a result, fluorescently labelled nucleotides are released and the resulting fluorescent signal is measured.
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