Method for quantification of neurotoxin

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United States of America

PATENT NO 8577618
SERIAL NO

12298908

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Abstract

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The present invention relates to a method for quantitatively measuring the muscular relaxing activity of a neurotoxin. Specifically, based on an extent of the activity of muscular relaxation of a neurotoxin from bacteria of Clostridium, the present invention relates to a method for quantification of the efficacy (potential and/or diffusion reaction) of a neurotoxin comprising the following steps of: (a) administering a neurotoxin to the hind leg muscle of one of hind legs of a non-human mammal; (b) applying electric stimulus to said non-human mammal; (c) measuring a compound muscle action potential (CMAP) by contraction of said hind leg muscle to which the neurotoxin is administered and/or of the hind leg muscle of the other hind leg to which the neurotoxin is not administered; and (d) taking amplitude data from the compound muscle action potential (CMAP) obtained by the measurement in step (c) and analyzing an extent of a decrease in amplitude to thereby quantify the efficacy of the muscular relaxing activity by the neurotoxin. In contrast to the mouse LD50 currently used as a potential unit of a botulinum toxin which is measurable at a level of only several units, the quantification method of the efficacy of a neurotoxin of the present invention allows for measurement at a level of as low as 0.01 to 1 unit and hence is a method with a high sensitivity, reproducibility and accuracy.

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Patent Owner(s)

  • THE CHEMO-SERO-THERAPEUTIC RESEARCH INSTITUTE;JAPAN AS REPRESENTED BY DIRECTOR GENERAL OF NATIONAL INSTITUTE OF INFECTIOUS DISEASES;JAPAN AS REPRESENTED BY DIRECTOR GENEREAL OF NATIONAL INSTITUTE OF INFECTIOUS DISEASES;JURIDICAL FOUNDATION THE CHEMO-SERO-THERAPEUTIC RESEARCH 'LNSTITL.YI'E

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Harakawa, Tetsuhiro Kumamoto, JP 3 7
Ishida, Setsuji Narashino, JP 1 5
Kaji, Ryuji Tokushima, JP 6 17
Nakano, Hirotoshi Kumamoto-ken, JP 2 7
Okuda, Sachio Kumamoto, JP 6 11
Sakamoto, Takashi Ichikawa, JP 222 1758
Takahashi, Motohide Musashimurayama, JP 5 49
Torii, Yasushi Kumamoto, JP 12 51

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