Apparatus for insulated isothermal polymerase chain reaction
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United States of America Patent
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Nov 5, 2013
Grant Date -
Sep 27, 2012
app pub date -
Dec 13, 2011
filing date -
Mar 22, 2011
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Abstract
An apparatus for holding a test tube in which insulated isothermal polymerase chain reaction is performed includes a heat sink and a tube rack. The heat sink has a main body with a through hole for insertion of the test tube. The through hole has a relatively big diameter section and a relatively small diameter section located below the relatively big diameter section. The tube rack is mounted on the heat sink for insertion of the test tube. The apparatus can ensure that the temperature at the liquid level of the reaction mixture is lower than the temperature suitable for conducting the primer annealing step in the polymerase chain reaction process.
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