Method for production of chromatography media
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United States of America Patent
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Nov 5, 2013
Grant Date -
Dec 6, 2012
app pub date -
Feb 16, 2011
filing date -
Feb 19, 2010
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Abstract
The present invention relates to a method of producing novel chromatography media and use thereof for purification of biomolecules, such as proteins. The chromatography media comprises shell beads having an inner porous core and an outer shell. The method comprises providing buffering ligands in the core of the beads, and providing binding ligands aimed for biomolecule binding in the outer shell of the beads. This method makes it possible to optimize binding properties and buffering properties independently of each other which is especially to advantageous for production of chromatofocusing media.
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